Upon March the 6th, 98067 cases of COVID-19 have been diagnosed worldwide. As a company focused on the development of innovative antibody drugs, Sanyou Biopharmaceuticals has obtained anti-virus monoclonal antibodies with neutralizing activity within just 16 days after the outburst, and has stocked a full range of high-quality 2019-nCoV-related proteins and mAbs with rigorous quality control and activity verification. In order to assist more researchers with their research of mechanism, development of diagnostic reagents, development of therapeutic drugs etc., a large number of antibodies are offered for free trial and related products now accept orders or pre-orders.

In order to assist more researchers with their mechanism research, development of diagnostic reagents, development of therapeutic drugs etc., related products now accept orders or pre-orders.

In the past month, we have provided 2019-nCoV protein and antibody products and services to more than 100 institutions or bio-technological companies in China.


Cat# Name Species Subtype/Label Expression System
Protein PNA001 Spike S1 SARS-CoV-2 Fc HEK293
PNA002 Spike S1 SARS-CoV-2 His HEK293
PNA003 Spike RBD SARS-CoV-2 Fc HEK293
PNA004 Spike RBD SARS-CoV-2 His HEK293
PNA005 Nucleocapsid Protein SARS-CoV-2 His E.coli
PNA006 Nucleocapsid Protein SARS-CoV-2 His HEK293
PHA001 ACE2 protein human Fc HEK293
PHA002 ACE2 protein human His HEK293
PMA001 ACE2 protein mouse Fc HEK293
Antibody AHA001 Anti-Spike-RBD mAb Human Human-IgG1 CHO
AHA003 Anti-Spike-RBD mAb Human Human-IgG1 CHO
AHA004 Anti-Spike-RBD mAb Human Human-IgG1 CHO
AHA013 Anti-Spike-RBD mAb Human Human-IgG1 CHO
AHA014 Anti-Spike-RBD mAb Human Human-IgG1 CHO
AHA005 Anti-Spike-RBD mAb Human Human-IgM CHO
ANA001 Anti-Spike-RBD single domain mAb Alpaca Fc(IgG) CHO
ANA002 Anti-Spike-RBD single domain mAb Alpaca Fc(IgG) CHO
ANA003 Anti-Spike-RBD single domain mAb Alpaca Fc(IgG) CHO
ANA004 Anti-Spike-RBD single domain mAb Alpaca Fc(IgG) CHO
AHA006 Anti-N-protein mAb Human Human-IgG1 CHO
AHA009 Anti-N-protein mAb Human Human-IgG1 CHO
AHA015 Anti-N-protein mAb Human Human-IgM CHO
AHA016 Anti-N-protein mAb Human Human-IgM CHO
AHA007 Anti-ACE2 mAb Human Human-IgG1 CHO



Our products are used for research only.If you have any question about inquiring, ordering or project progress, please send an email to service@sanyoubio.com, or call the toll-free customer service phone: +86 21 33681627. We will reply to you within 24 hours.


   2019-nCoV S1 protein


The 2019-nCoV S1 protein was expressed using the HEK293 expression system with more the 95% purity.


Fig.1 SDS-PAGE of 2019-nCoV S1 protein L1 is S1-Fc protein, L2 is S1-His protein




2019-nCoV S1 protein has excellent affinity to ACE2 protein or S1 protein antibody.


Fig. 2 ELISA binding test between ACE2 and 2019-nCoV S1

Fig. 3 ELISA binding between Anti-S1 antibody and 2019-nCoV S1


2019-nCoV S1 protein shows excellent affinity to Vero E6 cell surface ACE2.


Fig. 4 FACS binding test of 2019-nCoV S1 to ACE2 expressing Vero E6 cells



In order to verify the application potential of our antigen and antibody products as raw materials for immunodiagnosis, we used the 2019-nCoV S1 protein as a raw material to make a prototype of the IgG detection kit. The results show that the colloidal gold detection kit has successfully detected the anti-neo coronavirus IgG antibody in the positive quality control, and the sensitivity can reach 10ng.


Fig. 5 Colloidal Gold Test. The first row is the experimental group and the second row is the blank control group, IgG concentration decreases from left to right.



   2019-nCoV Spike-RBD protein


2019-nCoV Spike-RBD protein was expressed using HEK293 expression system with purity of 95%.


Fig. 6 SDS-PAGE of 2019-nCoV Spike-RBD. L1 is Spike RBD-Fc.


2019-nCoV S1 protein shows excellent affinity to Vero E6 cell surface ACE2.


Fig.7 FACS binding test of 2019-nCoV S1 to ACE2 expressing Vero E6 cells.




   2019-nCoV anti-Spike-RBD fully human mAb


2019-nCoV Anti-Spike-RBD fully human mAb was expressed using CHO expression system with purity of 95%.


Fig. 8 SDS-PAGE of anti-Spike-RBD human mAb L1 is control,L2 is anti-Spike-RBD human mAb


The anti-Spike-RBD human mAb shows prominent affinity to 2019-nCoV Spike-RBD protein.


Fig. 9 ELISA binding between Anti-Spike-RBD fully human mAb and Spike-RBD protein


The anti-Spike-RBD fully human mAb shows good blocking activity against the 2019-nCoV Spike-RBD protein.

Fig. 10 FACS binding test of Anti-Spike-RBD mAb and Spike-RBD protein to ACE2 expressing Vero E6 cells



In order to explore the matching of our monoclonal antibodies, we used the das-Elisa method for analysis. The results show that A1 and A3, A2 and A3 are validated paired antibodies against different epitopes.

Fig. 11 Double antibody sandwich Elisa. It shows the pre-bound S1 protein and A1 mAb, pre-bound S1 protein and A2 mAb added A3 monoclonal antibody detection.


   2019-nCoV Anti-Spike-RBD single domain mAb


Single domain mAbs naturally lack light chains. They have many advantages over traditional antibodies, such as small molecular weight, strong specificity, high affinity, and high expression. Sanyou Bio has screen several high-quality Anti-Spike-RBD Single domain mAbs using the company’s sub-Trillion Single Domain Antibody Library.


Fig. 12 SDS-PAGE of Anti-Spike-RBD single domain mAb



The Anti-Spike-RBD single domain mAb shows good blocking activity against the 2019-nCoV Spike-RBD protein.


Fig. 13 FACS binding test of  Anti-Spike-RBD single domain mAb and Spike-RBD protein to ACE2 expressing Vero E6 cells

   ACE2 proteins (human, murine)


Both human and murine ACE2 proteins were expressed using the HEK293 system, with the protein purity more than 95%.


Fig. 14 SDS-PAGE of ACE2proteins L1,L2 are human ACE2 proteins,L3,L4 are murine proteins



Human ACE2 protein has good affinity to 2019-nCoV S1 protein and anti-ACE2 antibody.


Fig. 15 ELISA binding of 2019-nCoV S1 and huACE2

Fig. 16 ELISA binding of anti-HuACE2 human mAb and huACE2

   2019-nCoV Anti-ACE2 human mAb


The Anti-ACE2 human mAb was expressed using the CHO expression system with more the 95% purity.


Fig.17 SDS-PAGE of Anti-ACE2 human mAb


The Anti-ACE2 human mAb has excellent affinity to ACE2 protein.


Fig.18 ELISA binding of Anti-ACE2 mAb and ACE2 protein

Anti-ACE2 mAb blocks the binding of ACE2 protein expressing Vero E6 cells.



Fig. 19 FACS binding test of ACE2 mAb to ACE2 expressing on Vero E6 cells


   2019-nCoV Anti-N-protein mAb


Sanyou Bio has screen several high-quality Nprotein human antibodies using the company’s sub-Trillion Human Naïve Antibody Library. 2019-nCoV Nprotein human antibody has excellent affinity to N protein.


Fig.20 ELISA binding of Anti-N-protein mAb and N protein



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